Development and Application of <i>Pi-k^h</i> Co-dominant Marker for Rice Blast Resistance Gene

Rice, as one of the staple food crops in world, is reduced by 10~30% every year due to damage rice blast.&nbsp;However, application marker assistant selection (MAS) with disease resistance genes can be effectively, accurately and rapidly used breed blast resistant varieties. The gene&nbsp; Pi-k h &nbsp;has been proved have broad-spectrum blast.&nbsp;Therefore, utilization of&nbsp; &nbsp;gene MAS breeding possess high value for improving resistance.&nbsp;In this study, based on two InDel differences Coding sequence (CDS) region &nbsp;gene, a co-dominant functional molecular was developed at conservative position sequence, which named ZJPikh.&nbsp;The susceptible variety &ldquo;Yudao 518&rdquo; female parent &ldquo;Shida 68&rdquo; male parent.&nbsp;The genotype &nbsp;related F2 isolated population generation plants were verified pedigree method MAS.&nbsp;A new third-grade 58&rdquo; with&nbsp; &nbsp;was successfully bred.&nbsp;At same time, the&nbsp; &nbsp;genotypes 21 main varieties identified, provided technical support targeted efficient carrying&nbsp; through technology. Our research provides convenience identifying germplasm resources marker-assisted breeding.